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目的:探讨运动及衰老对大鼠不同部位骨密度(Bonemineraldensity,BMD)的影响。方法:20只10月龄雌性大鼠根据体重进行配伍,随机率分为对照组和运动组。对照组大鼠正常活动,运动组大鼠在跑台上进行18周运动(18m/min,1h/day,5d/week)。分别在第0、6、12、18周用双能X线骨密度仪测定两组大鼠椎体和股骨BMD,比较两组大鼠不同部位不同时间点的BMD。结果:对照组大鼠椎体BMD在不同时间点有显著性差异,与第0周时的BMD相比,第6、12、18周时的椎体BMD值均显著降低。对照组大鼠股骨BMD在不同时间点有显著性差异,与第0周时的BMD相比,第12、18周股骨BMD值均显著降低。运动组股骨和椎体BMD在各个时间点比较无显著性差异。运动组与对照组在第0、6周时的股骨BMD无显著性差异;在第12、18周,运动组股骨BMD显著高于对照组。运动组与对照组在第0、6、12周时椎体BMD无显著性差异;第18周时,运动组椎体BMD显著高于对照组。结果表明,10月龄大鼠由于衰老发生骨量丢失。大鼠中轴骨反映衰老导致骨量丢失的敏感性高于四肢骨。运动可缓解由于衰老导致的骨量丢失,其作用在四肢骨比在中轴骨显著。骨质疏松实验动物研究应根据不同目的选择研究部位。
Objective: To investigate the effect of exercise and aging on the bone mineral density (BMD) in different parts of rats. Methods: Twenty female 10-month-old rats were matched according to body weight. The randomization rate was divided into control group and exercise group. The rats in the control group were in normal activity. The rats in the exercise group exercised for 18 weeks (18m / min, 1h / day, 5d / week) on the treadmill. BMD of vertebral body and femur were measured by dual energy X-ray absorptiometry at the 0th, 6th, 12th and 18th week respectively. The BMD of different parts of the two groups were compared at different time points. Results: The BMD of vertebral body in control group was significantly different at different time points. Compared with BMD at 0 week, the BMD of vertebral body at 6th, 12th and 18th week were significantly decreased. The BMD of femur in control group was significantly different at different time points. Compared with BMD at 0 week, BMD of femur decreased significantly at 12th and 18th week. BMD of femur and vertebral body in exercise group had no significant difference at all time points. There was no significant difference in femur BMD between exercise group and control group at 0 and 6 weeks. BMD in femur of exercise group at 12th and 18th week was significantly higher than that of control group. There was no significant difference in BMD of vertebral bodies between exercise group and control group at 0, 6 and 12 weeks. BMD of exercise group at 18th week was significantly higher than that of control group. The results showed that 10-month-old rats lost bone mass due to aging. The sensitivity of the central axis of the rat to reflect bone loss caused by aging is higher than that of the extremities. Exercise can alleviate bone loss due to aging, its role in the limb bone than in the axial bone significantly. Osteoporosis experimental animal research should be selected according to different purposes research site.