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应用定量溶血分光光度测定法（ＱＨＳ）检测５－羟色胺（５－ＨＴ）对大鼠体液免疫应答的影响，并应用高效液相色谱－电化学检测法（ＨＰＬＣ－ＥＣＤ）测定大鼠在体液免疫应答的高峰期，中枢和外周５－ＨＴ代谢的变化。结果表明：给大鼠腹腔注射５－ＨＴ的前体５－羟色氨酸（５－ＨＴＰ）后，动物对抗绵羊红细胞（ＳＲＢＣ）的体液免疫应答减弱；腹腔注射５－ＨＴ的合成抑制剂对氯苯丙氨酸（Ｐ－ＣＰＡ）后，免疫应答增强，在体液免疫应答的高峰日（免疫后第５天），下丘脑和脑干内５－ＨＴ的含量明显增加，５－ＨＴ的代谢产物５－羟吲哚乙酸（５－ＨＩＡＡ）的含量无显著变化；脾脏中５－ＨＴ的水平无明显升高，而５－ＨＩＡＡ含量显著增多；海马和胸腺内５－ＨＴ代谢没有明显改变。上述结果提示，５－ＨＴ与体液免疫应答之间存在着相互作用，即５－ＨＴ可能抑制免疫应答，而免疫应答又可加强中枢神经系统和外周胸腺、脾脏内５－ＨＴ的代谢。
The effect of 5-hydroxytryptamine (5-HT) on the humoral immune response in rats was detected by quantitative haemolysis spectrophotometry (QHS). The humoral immune response in rats was determined by HPLC-ECD Response peak, central and peripheral changes in 5-HT metabolism. The results showed that the humoral immune response against SRBC was attenuated in rats by intraperitoneal injection of 5-HT (5-HTTP), a 5-HT preconditioning agent. The intraperitoneal injection of a synthetic inhibitor of 5-HT The level of 5-HT in the hypothalamus and brainstem increased significantly on the peak day of humoral immune response (5 days after immunization), while the metabolism of 5-HT There was no significant change in the content of 5-HIAA in the spleen, 5-HIAA in the spleen but no significant change in the 5-HIAA content in hippocampus and thymus. The above results suggest that there is an interaction between 5-HT and the humoral immune response, that is, 5-HT may suppress the immune response, which in turn enhances the metabolism of 5-HT in the central nervous system, peripheral thymus and spleen.