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目的研究藏药“嘎布得罗”(毛翠雀全草)中的化学成分,为其药效成分及作用机制深入研究奠定基础。方法采用甲醇提取、不同极性有机溶剂萃取,获得氯仿萃取部位;采用正相硅胶、反相硅胶、Sephadex-20等现代色谱技术进行分离纯化,综合运用质谱(MS)及核磁共振光谱(1H-NMR、13C-NMR)等现代波谱学方法和技术进行化合物的结构鉴定。结果从毛翠雀的氯仿部位分离得到5个含氮有机化合物,其中3个二萜类生物碱:2-羟基-11,13-二乙酰基海替生碱(1)、hetisinone(2)和browniine(3);1个阿朴菲类生物碱:异波尔定碱(4);腺苷(5)。结论化合物4和5为首次从该属植物中分离得到,全部5个化合物均为首次从该植物中分离得到。
Objective To study the chemical constituents in Tibetan medicine “Gabudoluo” (Mao del finches), and lay the foundation for its further study on its pharmacodynamics and its mechanism. Methods Methanol extraction and organic solvent extraction with different polarities were used to obtain the extraction sites of chloroform. The compounds were isolated and purified by using modern chromatographic techniques, such as normal phase silica gel, reverse phase silica gel and Sephadex-20, and mass spectrometry (MS- NMR, 13C-NMR) and other modern spectroscopic methods and techniques for structural identification of compounds. Results Five nitrogen-containing organic compounds were isolated from the chloroform fraction of P. delbrueckii. Among them, three diterpenoid alkaloids, 2-hydroxy-11,13-diacetylhodotenoid (1), hetisinone browniine (3); 1 apoflora alkaloid: isoporintine (4); adenosine (5). Conclusion Compounds 4 and 5 were isolated from this genus for the first time, and all 5 compounds were isolated from this plant for the first time.