用自制单碳纤维电极,配台微分脉冲伏安法(differential pulse voltammetry,DPV),在体外标准液中可获得一个能与抗坏血酸和5-羟吲哚乙酸完全分离的3,4双羟苯乙酸(DOPAC)峰。此蜂的高度在一定范围内与DOPAC的浓度成直线关系(r=0.991)。在麻醉大鼠尾核,也可测得明显的DOPAC峰,并且当刺激内侧前脑束后,此峰明显升高。用DPV和高压液相色谱法同时作麻醉家兔侧脑室脑脊液检测,发现腹腔注射多巴前后,两种方法得出的DOPAC变化时程非常相似。 A homemade single carbon fiber electrode with differential pulse voltammetry (DPV) was used to obtain 3,4-dihydroxyphenylacetic acid (3,4-dihydroxyphenylacetic acid) completely separated from ascorbic acid and 5- DOPAC) peak. The height of this bee had a linear relationship with DOPAC concentration (r = 0.991) within a certain range. In the caudate nucleus of anesthetized rats, significant DOPAC peaks were also measured, and this peak was significantly elevated when the medial forebrain bundle was stimulated. DPV and high pressure liquid chromatography for simultaneous detection of cerebrospinal fluid in the lateral ventricle of anesthetized rabbits, found that intraperitoneal injection of dopa before and after the two methods of DOPAC changes in the time course is very similar.