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目的:探讨玉郎伞多糖(YLSPS)体外对BALB/C小鼠淋巴细胞、巨噬细胞分泌肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、γ-干扰素(IFN-γ)及白细胞介素-2(IL-2)的影响及作用机制。方法:分离BALB/C小鼠腹腔巨噬细胞和脾淋巴细胞,分为空白对照组、LPS或ConA对照组和各浓度YLSPS组(50,100,200 mg·L~(-1)),MTT法检测脾淋巴细胞增殖,ELISA法检测细胞培养液中TNF-α、IL-6、IFN-γ、IL-2的浓度,RT-PCR法检测TNF-α、IL-6、IFN-γ、IL-2 mRNA的表达。结果:各浓度YLSPS组能剂量依赖性地促进BALB/C小鼠脾淋巴细胞增殖和TNF-α、IL-6、IFN-γ、IL-2的分泌,显著增强这些细胞因子的mRNA的表达(P<0.01或P<0.05)。结论:YLSPS体外能促进BALB/C小鼠脾淋巴细胞和腹腔巨噬细胞分泌IFN-γ、IL-2、TNF-α和IL-6,其作用机制可能与上调上述细胞因子mRNA表达有关。
Objective: To investigate the effects of YLSPS on the secretion of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interferon-γ in BALB / c mice lymphocytes and macrophages (IFN-γ) and interleukin-2 (IL-2) and its mechanism of action. Methods: Peritoneal macrophages and splenic lymphocytes were isolated from BALB / c mice and divided into blank control group, LPS or ConA control group and YLSPS group (50,100,200 mg · L -1) The cell proliferation was measured by ELISA. The concentrations of TNF-α, IL-6, IFN-γ and IL-2 in cell culture medium were detected by ELISA. The mRNA expression of TNF-α, IL-6, IFN-γ and IL- expression. Results: YLSPS at various concentrations could promote the proliferation of splenic lymphocytes and the secretion of TNF-α, IL-6, IFN-γ and IL-2 in BALB / C mice in a dose-dependent manner and significantly enhance the mRNA expression of these cytokines P <0.01 or P <0.05). CONCLUSION: YLSPS can promote the secretion of IFN-γ, IL-2, TNF-α and IL-6 from spleen lymphocytes and peritoneal macrophages in BALB / c mice in vitro. The mechanism may be related to the up-regulation of the above cytokine mRNA expression.