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目的:探讨甲状腺乳头状癌(PTC)中PI3K mRNA、Akt mRNA、PI3K和Akt的表达与意义。方法:采用逆转录聚合酶链反应(RT-PCR)测定组织中PI3KmRNA和Akt mRNA的表达;应用免疫组化法测定组织中PI3K和Akt蛋白的表达。结果:PTC中PI3KmRNA表达量(90.3±5.06)和Akt mRNA的表达量(78.13±5.5)高于癌旁正常甲状腺组织(NAT)表达量37.74±10.63和14.82±5.63,P<0.05。免疫组化结果显示,甲状腺乳头状癌PI3K蛋白表达阳性率73.33%、Akt蛋白表达阳性率(83.33%)明显高于NAT组织蛋白表达阳性率33.33%和16.67%,P<0.05。PTC中PI3K和Akt蛋白表达与包膜浸润及淋巴结转移相关,P<0.05。结论:PTC中存在PI3K/Akt信号传导通路的异常激活,信号传导途径的激活水平与PTC浸润和局部转移有关。
Objective: To investigate the expression and significance of PI3K mRNA, Akt mRNA, PI3K and Akt in papillary thyroid carcinoma (PTC). Methods: The expression of PI3K mRNA and Akt mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expressions of PI3K and Akt protein were detected by immunohistochemistry. Results: The expression of PI3K mRNA (90.3 ± 5.06) and Akt mRNA (78.13 ± 5.5) in PTC tissues were significantly higher than those in adjacent normal thyroid tissues (37.74 ± 10.63 and 14.82 ± 5.63, P <0.05). Immunohistochemistry showed that the positive rate of PI3K protein in thyroid papillary carcinoma was 73.33%, the positive rate of Akt protein was 83.33%, which was 33.33% and 16.67% respectively (P <0.05). The expression of PI3K and Akt in PTC was correlated with the infiltration and lymph node metastasis (P <0.05). Conclusion: PI3K / Akt signaling pathway is abnormally activated in PTC. The activation of signal transduction pathway is related to PTC infiltration and local metastasis.