论文部分内容阅读
用甘蓝型油菜(Brassica napus L.)7055为材料,进行五种处理的低温冷藏,以研究低温冷藏对油菜苔段分化和绿苗成活率的影响。结果证明,以消毒后的苔段,低温冷藏后再切段分化培养,效果最佳。低温冷藏时间长达90天,仍不影响苔段分化,可安全越夏。其余处理冷藏时间一般不能超过60天。分化的绿苗冷藏,更不能超过60天,经60天冷藏后可供移栽的实用苗数则下降50％。通过这一研究,对油菜试管苗的安全越夏、正常适宜时节种植、在育种上的利用、无性快速繁殖和种质保存,都有一定意义。
Cold storage of five treatments was conducted with Brassica napus 7055 as a material to study the effects of cold storage on the differentiation of rape moss and the survival rate of green shoots. The results show that, after disinfection of the moss, cold storage and then cut into sections of culture, the best. Cold storage time up to 90 days, still does not affect the moss of differentiation, safe summer. The rest of the handling of refrigeration time generally can not exceed 60 days. Differentiated green seedlings refrigerated, but can not exceed 60 days, 60 days after cold storage for practical transplanting seedlings decreased by 50%. Through this study, the rape plantlets safe summer, the normal planting season, the use of breeding, asexual rapid propagation and conservation of germplasm, have a certain significance.