0　引言　现有的半薄切片多用甲苯胺蓝 [1 ] 及碱性复红染色 ,但在观察时往往组织结构、细胞核及细胞质分辨不够清楚 .此法是在半薄切片染色改良法 [2 ] 的基础上 ,经反复实验 ,进一步改进而成的 .本法操作简单 ,染色效果良好而且着色均匀 ,组织结构清晰 ,适用于电子显微镜超薄 0 Introduction The existing semi-thin sections with toluidine blue [1] and alkaline complex red staining, but often observed in the organization structure, the nucleus and cytoplasm is not clear enough to distinguish this method is in the semi-thin section staining method [2] On the basis of repeated experiments and further improvements made.The method is simple, good dyeing effect and uniform coloring, clear organizational structure, suitable for ultra-thin electron microscope