Aim:To obtain the recombinant,VPAC2-selective ( VPAC2:type 3 receptor ofpituitary adenylate cyclase activating polypeptide which shared by vasoactiveintestinal peptide) agonist with effects on glucose disposal by intein-mediated,single column purification.Methods:A gene encoding 32-amino acid peptidenamed rMBAY was designed and synthesized and cloned into Escherichia coliexpression vector,pKYB (NEB,USA).The recombinant vector was transferredinto E coli ER2566 strain and the target protein was overexpressed as a fusion tothe N-terminus of a self-cleavable affinity tag.After the fusion protein was puri-fled by chitin-affinity chromatography,the self-cleavage activity of the intein wasinduced by β-mercaptoethanol and the target peptide,rMBAY,was released fromthe chitin-bound intein tag.Results:Approximately 53 mg rMBAY with the purityover 95% was obtained by single column purification from 1 L induced culturefermented in 5 L fermenter.The results of the competitive binding assay andcAMP accumulation assay indicated that the recombinant rMBAY had specialbinding selectivity and potency for VPAC2.The recombinant peptide,rMBAY,enhanced insulin release and decreased the plasma glucose level after intraperito-neal injection (50 ng/kg) with a high concentration of glucose (1.8 mmol/kg) in theNIH mice.Conclusion:An efficient production procedure of a recombinant VPAC2-selective agonist with corresponding effects on glucose disposal was established. Aim: To obtain the recombinant, VPAC2-selective (VPAC2: type 3 receptor ofituitary adenylate cyclase activating polypeptide which shared by vasoactive intestinal peptide) agonist with effects on glucose disposal by intein-mediated, single column purification. Methods: A gene encoding 32-amino acid peptidenamed rMBAY was designed and synthesized and cloned into Escherichia coliexpression vector, pKYB (NEB, USA). The recombinant vector was transferred in E. coli ER2566 strain and the target protein was overexpressed as a fusion tothe N-terminus of a self-cleavable affinity tag . After the fusion protein was puri-fled by chitin-affinity chromatography, the self-cleavage activity of the intein was induced by β-mercaptoethanol and the target peptide, rMBAY, was released from the chitin-bound intein tag. Results: Approximately 53 mg rMBAY with the purity of 95% was obtained by single column purification from 1 L induced culture fermented in 5 L fermenter. The results of the competitive binding assay and cAMP accumulation assay indicated that the recombinant rMBAY had special binding selectivity and potency for VPAC2.The recombinant peptide, rMBAY, enhanced insulin release and decreased the plasma glucose level after intraperito-neal injection (50 ng / kg) with a high concentration of glucose / kg) in the NIHH mice. Confc: An efficient production procedure of a recombinant VPAC2-selective agonist with corresponding effects on glucose disposal was established.