在国内首次研究了贯叶连翘胚轴和子叶的培养方法，分别通过器官型和器官发生型途径获得了大量再生植株，部分可移栽成活。实验表明，在ＢＡ和２，４－Ｄ不同组合的ＭＳ培养基上，愈伤组织易于诱导，繁殖速度快，并且通过初步检测证明了愈伤组织中金丝桃素存在的可能性。再生植株产生极容易，繁殖系数高。 In China, the culture method of hypocotyl and cotyledon of Hypericum perforatum was first studied in this paper. A large number of regenerated plants were obtained through organ and organogenesis respectively, and some of them were transplanted and survived. Experiments showed that the callus was easy to induce and reproduce fast on the MS medium with different combinations of BA and 2,4-D, and the initial detection proved the possibility of the existence of the hypericin in the callus. Regeneration plants produce very easy, high breeding coefficient.