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采用目前公认的 DN A氧化损伤标志物 8- OHd G的单抗 ,利用 L SAB法研究不同年龄组 BAL B/ C小鼠胸腺和脾脏8- OHd G的生成水平 ,对免疫组织在衰老过程中 DNA氧化损伤的水平进行免疫组织化学研究 ,以探讨免疫系统的自由基损伤对衰老的影响。结果表明 ,在胸腺 ,8- OHd G+细胞密度随增龄而增多 ,并主要位于髓质区 ;脾脏中的阳性细胞则无明显的增龄性变化 ,但胞染形态却存在明显差异。本研究结果显示 ,衰老过程中 ,免疫细胞内 8- OHd G含量发生改变 ,免疫系统受到了氧自由基的损伤。 8- OH d G可作为免疫老化过程中的一种生物标志
Using currently recognized monoclonal antibody 8-OHd G, an oxidative damage marker of DN A, L-SAB was used to study the generation of 8-OHd G in thymus and spleen of BAL B / C mice of different age groups. DNA oxidative damage levels of immunohistochemistry to investigate the immune system of free radical damage on aging. The results showed that in the thymus, the density of 8-OHd G + cells increased with increasing age, and mainly located in the medulla. The positive cells in the spleen showed no obvious age-related changes, but there were significant differences in the morphology of the cells. The results of this study show that during the aging process, the content of 8-OHd G in immune cells changes, and the immune system is damaged by oxygen free radicals. 8-OH d G can be used as a biomarker during immune aging