论文部分内容阅读
目的 :用软件预测日本血吸虫重组 2 8GST抗原分子T细胞表位谱 ,并鉴定其Th1型细胞表位。方法 :用软件预测重组2 8GSTT细胞表位谱 ,并筛选几种较好的T细胞表位 ,人工合成表位肽或用基因工程制备重组表位肽融合蛋白。体外刺激经照射的尾蚴感染并用 2 8GST加强免疫的C5 7BL/ 6小鼠 (H 2 b)脾细胞 ,通过淋巴细胞增殖试验、ELISA及流式细胞术等 ,分析各种表位的免疫刺激作用 ,鉴定Th1型细胞表位。结果 :在 9个候选的表位中 ,P6 (73～ 86aa)是刺激作用最强的Th1型细胞表位。结论 :重组 2 8GST含有功能性Th1型细胞表位
Objective: To predict the T cell epitopes of recombinant 2 8GST antigen of Schistosoma japonicum by software and identify its Th1-type cell epitopes. Methods: The epitopes of recombinant 2 8GSTT cells were predicted by software and several good T cell epitopes were screened. Synthetic epitope peptide was synthesized or recombinant epitope peptide fusion protein was prepared by genetic engineering. C5 7BL / 6 mouse (H 2 b) splenocytes infected with irradiated cercariae and boosted with 2 8 GST were stimulated in vitro and the immunostimulatory effects of various epitopes were analyzed by lymphocyte proliferation assay, ELISA and flow cytometry , Identifying Th1-type cell epitopes. Results: Of the nine candidate epitopes, P6 (73-86aa) was the most stimulatory Th1-type cell epitope. Conclusion: Recombinant 28GST contains functional Th1-type cell epitopes