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从大鼠肝分离出小分子蛋白质,注入肝大部切除的大鼠体内。术后24h制备肝细胞悬液及骨髓细胞悬液,用流式细胞计测定细胞DNA含量。结果:①实验组大鼠每克体重注入肝提取物10～300μg,一致呈现4n肝细胞DNA复制受阻于G_1期,各类肝细胞数与正常大鼠的相似;②对照组大鼠,4n肝细胞由正常的68.1％降至30.9％,8n肝细胞由6.3％骤增至25.3％,并出现16.8％的4n～8n间8期肝细胞;③两组大鼠的骨髓细胞DNA含量均无变化。表明肝提取物中含有肝抑素样物质,它特异性地使再生肝的4n肝细胞增殖周期阻滞于G_1期及S早期。
Small-molecule proteins were isolated from rat livers and injected into rats with major hepatectomy. Hepatocyte suspensions and bone marrow cell suspensions were prepared 24 h after surgery, and the cell DNA content was measured by flow cytometry. RESULTS: 1 Rats in experimental group were injected with 10-300 μg of liver extract per gram of body weight, which consistently showed that 4 n hepatocyte DNA was blocked from replicating in G 1 phase, and the number of hepatocytes was similar to that of normal rats; 2 rats in control group, 4 n liver The number of cells from the normal 68.1% to 30.9%, 8n hepatocytes increased from 6.3% to 25.3%, and 16.8% of 4n ~ 8n between the eight phases of hepatocytes; 3 no changes in the DNA content of bone marrow cells in both groups of rats . The hepatic extract contained heparin-like substance, which specifically blocked the proliferation cycle of 4n hepatocytes in the regenerating liver in the G 1 and early S phases.