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自20世纪90年代初至今,布鲁杆菌病(简称布病)在世界范围内出现了疫情回升的趋势,成为再度肆虐的传染病。优良的诊断技术是布病防治的先决条件。随着分子生物学技术不断成熟和发展,已广泛应用于感染性疾病的诊断和其他分子生物学研究领域,开辟了布病及布鲁杆菌污染物早期诊断和检测的新途径。1核酸探针检测Mater等[1]用PCR扩增编码3l k Da抗原的基因序列,制备地高辛标记探针,鉴定布鲁杆菌属。这一序列
Since the early 1990s, brucellosis (brucellosis) has seen a worldwide epidemic of rebound and has become a recurrent epidemic. Good diagnostic techniques are a prerequisite for the control of brucellosis. With the continuous maturation and development of molecular biology techniques, it has been widely used in the field of infectious disease diagnosis and other molecular biology research, opening up new ways for the early diagnosis and detection of brucellosis and brucella contamination. 1 Nucleic Acid Probe Detection Mater et al [1] amplified the gene sequence encoding the 3l kDa antigen by PCR, and prepared digoxigenin labeled probe to identify Brucella. This sequence