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目的了解四川省预做脐带血保存的孕产妇标本核酸检测情况,评价对预做脐带血保存的孕产妇标本进行核酸的意义。方法于2015年7-12月,收集预在四川省脐带血造血干细胞库做脐带血保存的孕产妇标本2管,先用酶联免疫法(ELISA)进行筛查,如果ELISA结果阳性,不再进行核酸检测;如果ELISA结果是阴性,则将其另一管标本送到输血研究所进行核酸检测。结果共收集到ELISA检测结果阴性标本4 878例,经核酸检测,共鉴别出HBV DNA阳性标本7例,无HIV RNA阳性和HCV RNA阳性结果。结论对于ELISA检测阴性的标本有必要进行核酸检测,对指导脐带血能否保存和避免输注脐带血传播病原体具有重要意义。
Objective To understand the detection of nucleic acid in maternal specimens preserved in cord blood in Sichuan Province and to evaluate the significance of nucleic acid in maternal specimens preserved in umbilical cord blood. Methods From July to December in 2015, 2 maternal samples of umbilical cord blood preserved in Sichuan cord blood hematopoietic stem cell collection were collected and screened by enzyme-linked immunosorbent assay (ELISA). If the ELISA result is positive, Nucleic acid test; if the ELISA result is negative, then send another tube specimens to the Transfusion Institute for nucleic acid detection. Results A total of 4 878 negative samples were collected. According to the results of nucleic acid tests, 7 HBV DNA positive samples were identified. There were no HIV RNA positive and HCV RNA positive results. Conclusion It is necessary to detect the nucleic acid for negative specimens by ELISA. It is of great significance to guide the cord blood to preserve and prevent the transfusion of pathogens from umbilical cord blood.