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目的 探讨嗜酸细胞 (EOS)在体内和体外呈递抗原的过程和特征。方法 以鸡卵清蛋白 (OVA)致敏和雾化吸入刺激BALB/c小鼠以诱发EOS在气道聚集后将其分离纯化。将曾在气道接触抗原的EOS和致敏T细胞在体外共同培养 ;同时 ,将EOS注入致敏小鼠气道 ,然后在预定的时间收集气管旁淋巴结细胞进行培养。观察由于EOS呈递抗原所引发T细胞在体外和体内的增殖反应的变化。结果 在体外实验中 ,如果没有EOS存在 ,T细胞不增殖。EOS数量逐渐增加之后 ,T细胞随即出现明显的增殖反应并呈数量相关性。在体内实验中 ,致敏小鼠气管内注入EOS 1天后气管旁淋巴结的T细胞增殖反应即有所增强 ,3天后达到峰值 ,1周以后下降。体内T细胞增殖反应随着注入气管的EOS数量的增加而增强。此外 ,无论是体内还是体外实验 ,暴露于某一抗原的EOS仅能将抗原呈递给经同一抗原致敏的T细胞。结论 EOS无论在体内还是在体外均能摄取和处理抗原 ,然后将抗原信息呈递给致敏T细胞促使后者增殖。并且 ,EOS呈递抗原的过程均表现出严格的抗原特异性
Objective To investigate the process and characteristics of eosinophils (EOS) presenting antigens in vivo and in vitro. Methods BALB / c mice were induced by inhalation of chicken ovalbumin (OVA) and aerosolized to induce EOS to separate and purify airway. The EOS and the sensitized T cells, which had been in contact with the airway antigen, were co-cultured in vitro; at the same time, EOS was injected into the airways of the sensitized mice, and then the para-tracheal lymph node cells were collected and cultured at a predetermined time. To observe the changes in the proliferative response of T cells induced by EOS presenting antigen in vitro and in vivo. Results In in vitro experiments, T cells did not proliferate in the absence of EOS. After the number of EOS gradually increased, T cells immediately after the proliferation reaction and a significant correlation. In in vivo experiments, T cells proliferated in the paratracheal lymph nodes of sensitized mice after intratracheal instillation of EOS for 1 day. That is to say, the T cell proliferative response increased to the peak at 3 days and decreased after 1 week. In vivo T cell proliferation response increases with the number of EOS injected into the trachea. In addition, either in vivo or in vitro experiments, EOS exposed to an antigen can only present the antigen to T cells sensitized with the same antigen. Conclusion EOS can both ingest and process antigens in vivo and in vitro, and then present the antigen information to sensitized T cells for proliferation. Moreover, the process of EOS antigen presentation showed strict antigen specificity