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背景:透明质酸具有与受体特异结合的特性,将其与阿霉素联合制成纳米粒,可以充分发挥药物靶向作用,降低药物的毒副作用。目的:探讨阿霉素-透明质酸纳米颗粒于体外靶向杀伤口腔鳞状细胞癌的作用。设计、时间及地点:体外对比观察实验,于2008-01/07在中国海洋大学药理学实验室完成。材料:人舌癌T8113细胞株由上海第九人民医院陈万涛教授惠赠,阿霉素-透明质酸纳米粒由中国海洋大学海洋生命学院惠赠。方法:在体外培养的口腔鳞状细胞癌细胞中分别加入阿霉素质量浓度为0.5,1.0,5.0,10.0mg/L的阿霉素-透明质酸纳米颗粒,利用体外细胞毒实验四甲基偶氮唑蓝法检测阿霉素-透明质酸纳米颗粒对肿瘤细胞的靶向杀伤作用;阿霉素质量浓度为5.0,10.0mg/L时分别作用0,6,12,24,48h后,利用流式细胞仪检测其对肿瘤细胞凋亡的作影响。主要观察指标:①细胞生存率。②细胞凋亡率。结果:24,48h细胞毒试验阿霉素-透明质酸纳米颗粒对肿瘤细胞的杀伤作用优于游离阿霉素(t=5.78～42.05,P<0.01)。相同浓度阿霉素-透明质酸纳米颗粒细胞凋亡百分率随时间增加而升高(F=4200.40,4775.36,P<0.01),相同作用时间凋亡率随浓度增加而升高(t=12.06～20.08,P<0.05)。结论:阿霉素-透明质酸纳米颗粒可特异性识别肿瘤细胞,并实现其杀伤作用,并随时间延长增加杀伤作用。
BACKGROUND: Hyaluronic acid has the property of binding specifically with receptors. Combined with doxorubicin, nanoparticles can be made into nanoparticles to fully exert the drug targeting effect and reduce the side effects of drugs. Objective: To investigate the effect of doxorubicin-hyaluronic acid nanoparticles targeting and killing oral squamous cell carcinoma in vitro. DESIGN, TIME AND SETTING: In vitro comparative observation was performed at the Pharmacology Laboratory, Ocean University of China, 2008-01 / 07. MATERIALS: Human tongue cancer T8113 cell line was kindly donated by Professor Chen Wantao of Shanghai Ninth People’s Hospital. Adriamycin-hyaluronic acid nanoparticles were kindly donated by Ocean Life College of Ocean University of China. METHODS: Adriamycin-loaded hyaluronic acid nanoparticles with adriamycin concentrations of 0.5, 1.0, 5.0 and 10.0 mg / L were respectively added into oral squamous cell carcinoma cells in vitro. Tetramethyl Azithromycin blue method for the doxorubicin - hyaluronic acid nanoparticles targeting tumor cell killing effect; doxorubicin mass concentration of 5.0, 10.0mg / L were 0,6,12,24,48 h, respectively, Flow Cytometry was used to detect the effect of apoptosis on tumor cells. MAIN OUTCOME MEASURES: ① cell survival rate. ② apoptosis rate. RESULTS: The cytotoxicity of doxorubicin-hyaluronic acid nanoparticles on tumor cells was better than that of free doxorubicin at 24h and 48h (t = 5.78-42.05, P <0.01). The percentage of apoptotic cells in the same concentration of doxorubicin-hyaluronic acid nanoparticles increased with time (F = 4200.40, 4775.36, P <0.01), and the apoptotic rate increased with increasing concentration (t = 12.06 ~ 20.08, P <0.05). CONCLUSION: Adriamycin-hyaluronic acid nanoparticles can specifically recognize tumor cells and achieve their killing effect, and increase the killing effect with time.