采用CPBA和RIA方法观察(±),(+),(-)棉酚对大鼠离体睾丸间质细胞的作用,结果表明:(±)和(-)棉酚可明显抑制间质细胞的AC活性,(-)棉酚抑制作用明显大于(±)棉酚;两者均能明显降低间质细胞cAMP、PGE和睾丸酮的含量,降低cAMP/cGMP,PGE/PGF的比值。(+)棉酚仅在高浓度时才可抑制间质细胞的AC活性及降低cAMP含量,而对PGE,睾丸酮的含量及cAMP/cGMP,PGE/PGF比值的影响则无显著性意义。 The effects of (+) and (-) gossypol on isolated rat testicular stromal cells were observed by CPBA and RIA methods. The results showed that (±) and (-) gossypol could significantly inhibit the AC and activity of (-) gossypol were significantly higher than that of (±) gossypol. Both of them could significantly decrease the content of cAMP, PGE and testosterone, and decrease the ratio of cAMP / cGMP and PGE / PGF. (+) Gossypol inhibited the AC activity and cAMP content of stromal cells only at high concentration, but had no significant effect on the content of PGE, testosterone and the ratio of cAMP / cGMP and PGE / PGF.