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该文以戊二醛为交联剂，将黄嘌呤氧化酶直接固定在丝蛋白做孔膜上，以平面圆盘铂电极为基础电极，研制成黄嘌呤氧化酶电极，检测酶催化反应中过氧化氢的产生量．该电极用酶量极少，对次黄嘌呤的检测下限达１×１０－７ｍｏｌ／Ｌ．用此电极对鱼新鲜度进行了测定，与食品分析中测定鱼类新鲜度的常规方法──挥发性盐基氮法和细菌菌落总数法比较，发现存在着很好的相关性，并且初步提出了以次黄嘌呤和黄嘌呤总量为指标判断鱼类新鲜度的范围．
In this paper, glutaraldehyde as cross-linking agent, the xanthine oxidase directly fixed in the silk protein membrane to do the hole, with a flat disk platinum electrode as the base electrode, developed xanthine oxidase electrode, detection of enzyme-catalyzed reaction The amount of hydrogen peroxide produced. The electrode with very little enzyme, hypoxanthine detection limit of 1 × 10-7mol / L. The electrode was used to determine the freshness of fish. It was found that there was a good correlation with the conventional method of determining the freshness of fish in food analysis - the volatile basic nitrogen method and the total number of bacterial colonies. To the total amount of hypoxanthine and xanthine as an indicator of freshness of the fish range.