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1978年初,我们与围场县菌肥厂一起进行了松苗菌根菌的试验研究。经过四年的试验,取得了一定的成果,现将研究、制作和使用情况介绍如下:一、菌根菌的分离培养菌根菌的分离用的是稀释法。取苗圃重茬土和大田土各1克,用灭菌水各稀释到1/亿。两种稀释液各是100毫升。再把这两种稀释液各取1毫升,放入盛有土豆培养基的培养皿内。培养基的温度为40℃,PH值为6—6.5。每个培养皿内加金霉素半片(研成
In early 1978, we conducted a pilot study of pine seedling mycorrhizal fungi with the Weichang County Fertilizer Plant. After four years of experiments, and achieved some results, now the research, production and use of the introduction are as follows: First, the separation and cultivation of mycorrhizal mycorrhizal bacteria is the use of dilution method. Take nursery reclaimed soil and field soil each 1 g, diluted with sterile water to 1/100 million. The two dilutions are 100 ml each. Then take these two dilutions each take 1 ml, into the Petri dish containing potato medium. The temperature of the medium is 40 ° C and the pH is 6-6.5. In each dish add chlortetracycline half (research into