论文部分内容阅读
在pH8.0的Tris-HCl介质中,三磷酸腺苷(ATP)能诱导十六烷基三甲基溴化铵(CTAB)包被的正电金纳米颗粒(AuNPs)聚集,引起其表面等离子体共振吸收及等离子体共振散射变化.通过扫描电子显微镜、动态光散射及Zeta电位表征了金纳米颗粒的聚集,讨论了ATP与金纳米颗粒的结合模式,确定了三磷酸腺苷与正电金纳米颗粒的配位作用.在此基础上建立了表面等离子体共振吸收定量检测ATP的方法.方法的线性范围为4.0~80μmol/L,检出限为0.82μmol/L.相同浓度的ADP,AMP,UTP,CTP和GTP不干扰测定.方法用于人体尿样ATP的检测,回收率在94.4%~123%之间,相对标准偏差RSD小于2.5%.与传统ATP检测方法相比,该方法简单快速,选择性好.
In pH8.0 Tris-HCl medium, adenosine triphosphate (ATP) induced the aggregation of cetyltrimethylammonium bromide (CTAB) -positive gold nanoparticles (AuNPs), causing its surface plasmon resonance absorption And plasma resonance scattering changes.The aggregation of gold nanoparticles was characterized by scanning electron microscopy, dynamic light scattering and Zeta potential.The binding mode between ATP and gold nanoparticles was discussed and the coordination of adenosine triphosphate and gold nanoparticles was confirmed On the basis of this, a method for the quantitative determination of ATP by surface plasmon resonance absorption was established.The linear range of the method was 4.0 ~ 80μmol / L with the detection limit of 0.82μmol / L. The same concentrations of ADP, AMP, UTP, CTP and GTP Without interfering with the determination.Methods The method was applied to the detection of human urine ATP with the recoveries between 94.4% and 123% and the relative standard deviations (RSDs) less than 2.5% .Compared with the traditional ATP detection method, the method is simple, rapid and selective.