目的　研究rasp2 1蛋白在bcr ablp2 10蛋白抗凋亡信号传导通路中的作用。 方法　应用逆转录病毒载体pLXSN将反义N ras1cDNA转导到K 5 62细胞系中后 ,通过足叶乙甙对细胞进行诱导凋亡。结果　转导反义N ras1cDNA的K 5 62细胞内N rasp2 1表达下降。反义N ras转导细胞较对照细胞K 5 62易发生凋亡 ,对药物的敏感性显著增高。 结论　rasp2 1蛋白是bcr ablp2 10蛋白介导抗凋亡信号的重要的下游蛋白 ,阻断该蛋白的功能有可能成为治疗人类慢性髓性白血病的 1个选择点 Objective To investigate the role of rasp2 1 in the anti-apoptotic signaling pathway of bcr ablp2 10 protein. Methods After antisense N ras1 cDNA was transduced into K562 cell line by retroviral vector pLXSN, the cells were induced to apoptosis by etoposide. Results N rasp2 1 expression in K 5 62 cells transduced with antisense N ras1 cDNA decreased. The antisense Nsr transduced cells were more susceptible to apoptosis than the control cells K562 and their sensitivity to drugs was significantly increased. Conclusion rasp2 1 protein is an important downstream protein that bcr ablp2 10 mediates anti-apoptotic signal. Blocking the function of this protein may become a selection point for the treatment of human chronic myeloid leukemia