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探索数字减影血管造影(digital subtraction angiography,DSA)插管技术在恒河猴(Macaca mulatta)脑脊液动态采集中的应用。将28只恒河猴作为实验动物,随机分为实验组和对照组,每组14只,经左前肢静脉推注3%戊巴比妥钠溶液全麻后,常规剔除背部脊柱周围体毛、消毒,实验组从穿刺开始就在DSA技术下行腰椎间隙穿刺,对照组直接行腰椎间隙穿刺,将硬膜外麻醉导管经腰椎蛛网膜下腔送至枕大池并埋置泵。对不同穿刺方法的结果数据进行χ2检验;神经功能参照改良Tarlov评分标准,切口采用改良的切口愈合等级评分标准。实验组经腰椎间隙穿刺蛛网膜下腔全部成功;对照组8只穿刺成功,6只穿刺失败;所有动物埋置泵均能从泵中采集到无色透明的脑脊液;术后恒河猴均于当天恢复正常。在DSA技术下经腰椎间隙穿刺蛛网膜下腔并埋置泵,成功率高,操作简便、安全性好,可行性强;通过埋置的泵采集脑脊液,快速省时,可实现在动物清醒时、全生理状态下对脑脊液进行动态多次采集,有助于提高非人灵长类实验动物的福利,由于采样导管置于枕大池,采集到的脑脊液样品质量有保证,可用于研究缺血性脑卒中发生机制,满足检验需要,具有一定的实用价值。
To explore the application of digital subtraction angiography (DSA) cannulation in the dynamic acquisition of cerebrospinal fluid in Macaca mulatta. Twenty-eight rhesus monkeys were randomly divided into experimental group and control group, with 14 rats in each group. After general anesthesia with 3% pentobarbital sodium bolus via left forelimb vein, In the experimental group, the lumbar intervertebral puncture was performed in the DSA technique from the beginning of puncture. The control group was directly punctured by the lumbar intervertebral space, and the epidural anesthesia catheter was delivered to the occipital cistern via the lumbar subarachnoid space and the pump was embedded. Chi-square test was performed on the data of results of different puncture methods; the neurological function was based on the improved Tarlov score standard, and the incision was improved with the score of incision healing grade. In the experimental group, lumbar intervertebral space puncture of subarachnoid space was successful. In the control group, 8 punctures were successful and 6 puncture failed. All animals were embedded pump can collect colorless and transparent cerebrospinal fluid from the pump. After operation, Same day returned to normal. Under the DSA technique, the subarachnoid space is punctured through the lumbar intervertebral space and the pump is embedded. The successful rate is high, the operation is simple and convenient, the safety is good and the feasibility is strong. The cerebrospinal fluid collected by the embedded pump can be saved quickly when the animal is awake , The full physiological state of the cerebrospinal fluid dynamic multiple acquisition, help to improve the non-human primate experimental animal welfare, due to the sampling catheter placed in the cistern, collected samples of cerebrospinal fluid quality assurance can be used to study the ischemic Stroke mechanism to meet the testing needs, has a certain practical value.