论文部分内容阅读
目的：对粘性放线菌５５１９Ⅰ型菌毛进行分离和部分特性研究。方法：采用超声粉碎方法从粘放菌５５１９细胞壁上分离Ⅰ型菌毛，然后经１６００００×ｇ２４ｈ高速离心，硫酸铵沉淀和葡聚糖凝胶Ｇ１００过柱后取得纯化菌毛，并经电镜和ＳＤＳ－ＰＡＧＥ电泳检查。结果：粘放菌５５１９Ⅰ型菌毛大部分分子量为５３５７３．４ｕ，少量为３７６９９．８ｕ。经电镜检查证实为Ⅰ型菌毛。结论：通过超声，结合高速离心，沉淀和葡聚糖凝胶Ｇ１００过程可得到纯化的粘放菌５５１９Ⅰ型菌毛
Objective: To study the isolation and partial characterization of Actinomyces viscosus 5519 strain Ⅰ. Methods: Type Ⅰ pilus pili was isolated from the cell walls of Mycobacterium phlei 5519 by ultrasonic pulverization. Purified pili were obtained after high speed centrifugation at 160000 × g24h, ammonium sulphate precipitation and Sephadex G100 column. The purified pili were obtained by electron microscopy and SDS -PAGE electrophoresis. Results: Most strains of Staphylococcus aureus 5519 type Ⅰ were 53573.4u with a small amount of 37699.8u. Electron microscopy confirmed type Ⅰ pili. CONCLUSIONS: Purified myxobacteria 5519 type I pili were obtained by sonication, combined with high-speed centrifugation, precipitation and Sephadex G100