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温度对温敏不育水稻二九青Ｓ花粉育性的影响极为明显，在３０℃高温下，其花粉接近全不育；２０℃低温下花粉不育度亦高，但不及３０℃高温下显著。与原种二九青相比，高低温均使二九青Ｓ花药可溶性蛋白质含量明显增加，低温下更为显著；蛋白质ＳＤＳＰＡＧＥ图谱表明经３０℃处理的二九青Ｓ花药蛋白质组分比原种二九青少了３条谱带。安农Ｓ－１在高温下花药中无花粉发育；而花药可溶性蛋白质含量高于造温（２５℃）下；ＳＤＳＰＡＧＥ蛋白质图谱也表明高温下少１条谱带。
The effect of temperature on the pollen fertility of temperature-sensitive genic male sterile rice, Erjianqing, was extremely obvious. Pollen sterility was close to total sterility at high temperature of 30 ℃. The pollen sterility at high temperature of 20 ℃ was also lower than that at 30 ℃ . Compared with the original cultivar Erjiuqing, both the high and low temperature increased the content of soluble protein in the anther of Erjianqing S significantly, especially at low temperature. The protein SDSPAGE map indicated that the protein composition of Erjianqing anther at 30 ℃ Two nine youth less 3 bands. Anan S-1 anthers pollen development at high temperatures; and anther soluble protein content higher than the temperature (25 ℃); SDSPAGE protein profile also shows a high temperature less than 1 band.