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本文仿照人口服免疫程序用小鼠为模型对冻干口服霍乱ｒＢＳ－ＷＣ菌苗制品进行了安全及保护率评价。给ＫＭ小鼠口服三种不同剂量的ｒＢＳ－ＷＣ菌苗后，无发病，无体重减轻，无死亡现象，表明该菌苗安全，无毒副作用。免疫组与安慰剂组小鼠用肠结扎攻毒试验评价保护效果，两组小鼠用吴江－２及滨－４３活菌攻击后，免疫组显示良好保护作用（Ｐ＜０．０５）。此法可以用于口服型ｒＢＳ－ＷＣ菌苗的评价，但也存在需要动物数较多，个体间差异较大，手术较麻烦的问题。
This article modeled the human oral immunization program using mouse as a model of safety and protection rate of lyophilized oral cholera rBS-WC vaccine products. KM mice were orally administered three different doses of rBS-WC vaccine, no disease, no weight loss, no death, indicating that the vaccine safe, non-toxic side effects. Immunization group and placebo group mice with intestinal ligation challenge test to evaluate the protective effect of two groups of mice with Wujiang-2 and -43 live bacteria attack, the immune group showed good protective effect (P <0.05). This method can be used for oral rBS-WC vaccine evaluation, but there are also the need for more animals, large differences between individuals, the operation is more cumbersome problems.