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Objective: To optimize the technological conditions of ultrasonic-assisted extraction of Amomum longiligulare T.L. Wu polysaccharides and increase the yield of polysaccharides. Methods: The polysaccharide was prepared by water extraction and alcohol precipitation method, and the yield of polysaccharide was taken as an index. The effects of ultrasonic time, extraction times, ultrasonic temperature, and water-to-material ratio on polysaccharide yield were investigated through a single factor experiment. Combined experimental design and response surface analysis were used to optimize the extraction process of Amomum longiligulare T.L. Wu polysaccharides. Results: The optimum conditions of ultrasonic-assisted extraction were determined as follows: extraction time of 29 min, three extraction times, water bath extraction temperature of 68 ℃, water-to-material ratio of 15: 1; under these conditions, the polysaccharide yield was 10.69％. Conclusion: The results are close to the predicted values of the model. This optimization test is effective and feasible, and provides a reference for the related research of Amomum longiligulare T.L.Wu.