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目的建立大鼠慢性氟中毒模型,观察氟中毒对大鼠肝脏、肾脏抗氧化能力的影响。方法 40只Wistar大鼠随机分为4组,分别为空白对照组、低剂量染氟组、中剂量染氟组、高剂量染氟组。8周后麻醉处死,腹主动脉取血,检测血清氧化应激水平;RT-PCR检测肝脏和肾脏Nrf2、HO-1、Gpx-1的mRNA表达。结果慢性氟中毒大鼠血清氟、GSH-px、SOD水平降低、MDA水平升高。RT-PCR检测结果表明,氟中毒大鼠肝、肾组织Nrf2、HO-1 mRNA表达升高,Gpx-1 mRNA表达降低。结论慢性氟中毒引起大鼠肝、肾组织氧化应激,其可能是与氟中毒调控Nrf2及其下游HO-1、Gpx-1表达有关。
Objective To establish a chronic fluorosis model in rats and observe the effect of fluorosis on the antioxidant capacity in rat liver and kidney. Methods Forty Wistar rats were randomly divided into four groups: blank control group, low dose fluoride group, middle dose fluoride group and high dose fluoride group. After 8 weeks, the rats were killed by anesthesia and blood was taken from abdominal aorta to detect the level of serum oxidative stress. The mRNA expressions of Nrf2, HO-1 and Gpx-1 in liver and kidney were detected by RT-PCR. Results Serum levels of fluoride, GSH-px, SOD were decreased and MDA level was elevated in chronic fluorosis rats. The results of RT-PCR showed that the expression of Nrf2 and HO-1 mRNA and the expression of Gpx-1 mRNA in liver and kidney of fluorosis rats were decreased. Conclusion Chronic fluorosis causes oxidative stress in rat liver and kidney tissues, which may be related to the regulation of Nrf2 and its downstream HO-1 and Gpx-1 expression by fluorosis.