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目的探讨丁苯酞(NBP)对戊四氮(PTZ)点燃慢性癫痫大鼠的认知功能损害的干预作用。方法60只SD大鼠随机分为2组,分别为盐水对照组(n=18)和癫痫模型组(n=42)。模型组以PTZ 35 mg·kg~(-1)·d~(-1)腹腔注射21 d造成慢性癫痫大鼠模型后,再随机分为PTZ组(n=18)和NBP组(n= 18),在继续给予PTZ注射的同时,NBP组给予NBP(80:mg·kg~(-1)·d~(-1))灌胃21 d,停药后采用Morris水迷宫、跳台和事件相关电位P300的方法观察。PTZ点燃癫痫模型学习记忆功能的改变、脑电生理的变化以及NBP的影响作用。结果PTZ组水迷宫中逃避潜伏期延长(P<0.01),平台象限游泳时间减少(P< 0.01),穿越平台次数减少(P<0.05);跳台试验中反应时间延长(P<0.01),步下潜伏期缩短(P< 0.01),错误次数增加(P<0.01);P300峰潜伏期延长(P<0.05)。在水迷宫和跳台测试初期,NBP组与对照组相比有明显差异(P<0.05),但在测试后期无明显区别(P>0.05),P300峰潜伏期无差异。结论PTZ点燃的慢性癫痫大鼠有空间学习记忆能力等认知功能的障碍,丁苯酞可部分改善慢性癫痫大鼠在水迷宫和跳台试验中的空间学习能力,明显改善空间记忆能力,并延长P300潜伏期,显著提高了癫痫大鼠的认知功能。
Objective To investigate the effects of butylphthalide (NBP) on cognitive impairment induced by pentylenetetrazole (PTZ) in chronic epileptic rats. Methods Sixty SD rats were randomly divided into two groups: saline control group (n = 18) and epilepsy model group (n = 42). The model group was induced by intraperitoneal injection of PTZ (35 mg · kg -1 · d -1) for 21 days. The model rats were randomly divided into PTZ group (n = 18) and NBP group (n = 18) ). NBP group was administered with NBP (80 mg · kg -1 · d -1) for 21 days while PTZ injection was continued. Morris water maze was used after withdrawal, and the platform and event-related Potential P300 method of observation. Changes of learning and memory function, changes of brain electrophysiology and effects of NBP in PTZ-triggered epilepsy model. Results In the water maze of PTZ group, the escape latency was prolonged (P <0.01), the swimming time in the platform quadrant was decreased (P <0.01), the number of traversing platforms was decreased (P <0.05) The incubation period was shortened (P <0.01), the number of errors was increased (P <0.01), and the latency of P300 peak was prolonged (P <0.05). There was a significant difference (P <0.05) between the NBP group and the control group at the beginning of the water maze and jumping test, but there was no significant difference at the later stage of the test (P> 0.05). There was no difference in the incubation period of the P300 peak. Conclusions PTZ-induced chronic epilepsy rats have cognitive dysfunction such as spatial learning and memory ability. Butylphthalide can partially improve the spatial learning ability in water maze and jumping test in chronic epileptic rats, and significantly improve the spatial memory ability and extend P300 latency significantly increased the cognitive function of epileptic rats.