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以往报道的抗细菌抗菌素及吞噬细胞在体外的相互作用均系在血清培养基进行实验,因此不能正确反映出多形核白细胞(PMN)对细菌的吞噬活性。鉴此,作者将已培育好的绿脓杆菌转用Hanks溶液进行如下观察: (1)抗菌素存在时,家兔PMN对绿脓杆菌的杀菌及吞噬活性: 抗绿脓抗菌素分两个系统进行。取PMN悬浮液4.8毫升,抗菌素溶液0.1毫升,绿脓杆菌悬浮液0.1毫升置于试管中(PMN为8×10~6/毫升,细菌为1×10~6/毫升)。另于对
Previously reported antibacterial and phagocyte interactions in vitro were performed in serum medium and therefore did not correctly reflect the phagocytic activity of PMN on bacteria. In view of this, the authors have been cultivated Pseudomonas aeruginosa transferred to Hanks solution for the following observations: (1) the presence of antibiotics, rabbit PMN bactericidal and phagocytic activity of Pseudomonas aeruginosa: anti-pus antibiotics in two systems. Take 4.8 ml of PMN suspension, 0.1 ml of antibiotic solution and 0.1 ml of Pseudomonas aeruginosa suspension in a test tube (PMN: 8 × 10-6 / ml, bacteria: 1 × 10-6 / ml). The other is right