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用苏云金芽孢杆菌ＨＤ－１、７２１６和ＣＴ－４３菌株晶体蛋白的碱溶和胰蛋白酶酶解产物作抗原，制备相应的抗血清。用环状沉淀反应、双相单扩散反应和火箭免疫电泳等技术测定了苏云金杆菌几个菌株和制剂的晶体蛋白的含量。用环状沉淀反应测定晶体蛋白的最低量为０．２２μｇ／ｍＬ，火箭免疫电泳为１５μｇ／ｍＬ，双相单扩散反应为６２μｇ／ｍＬ。生物测定的结果与几种血清学方法测定的结果有一定的相关性，菌株ＨＤ－１、７２１６和ＣＴ－４３的伴胞晶体经碱溶和胰蛋白酶处理后对小菜蛾幼虫的ＬＣ５０值分别为０．１４８、０．２４７和０．０９７μｇ／ｍＬ；经碱溶而未经胰蛋白酶处理的晶体蛋白对小菜蛾幼虫的ＬＣ５０值分别为１．８６７、３．１４７和１．０２２μｇ／ｍＬ，两种处理方法对晶体蛋白毒力影响很大。环状沉淀反应测定苏云金芽孢杆菌晶体蛋白含量灵敏度比火箭免疫电泳高，操作简便而快速；双相单扩散法灵敏度与火箭免疫电泳接近，但操作更简便
The corresponding antiserum was prepared using the alkaline solution of trypsin and the alkaline solution of the crystal proteins of Bacillus thuringiensis HD-1, 7216 and CT-43 as antigens. The crystal protein content of several strains and preparations of Bacillus thuringiensis was determined by using ring precipitation reaction, two-phase single-diffusion reaction and rocket immunoelectrophoresis. The lowest amount of crystal protein was 0.22μg / mL when measured by ring-like precipitation reaction, 15μg / mL by rocket immunoelectrophoresis and 62μg / mL by biphasic single-diffusion reaction. Bioassay results and several serological methods to determine the results of a certain correlation between strain HD-1, 7216 and CT-43’s companion crystal by alkali-soluble and trypsin treatment of diamondback moth larvae LC50 values were 0.148, 0.247 and 0.097 μg / mL, respectively. The LC50 values of crystal proteins without alkaline protease to Plutella xylostella larvae were 1.867, 3.147 and 1.022 μg / mL respectively, The treatment of crystal protein toxicity has a great impact. Ring precipitation reaction determination of Bacillus thuringiensis crystal protein sensitivity than the rocket immunoelectrophoresis, easy and fast; biphasic single-diffusion sensitivity and rocket immunoelectrophoresis approach, but the operation is more convenient