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本研究组对脊髓可塑性研究已证实，切除猫部分背根后，保留的背根（备用报）纤维可则侧支出芽代偿被切断的传入纤维，并与其靶区（背角）重建突触联系。用电泳和体外培养等方法亦发现备用背根猫去传入侧脊髓背角组织提取液中一些蛋白质的含量发生了变化，而且其促神经突起生长的作用增强。在以上研究的基础上，我们采用凝胶过滤层析方法，分离提取液中对神经突起生长有促进作用的相关蛋白质。实验采用成年雄性家猫30只，切除一侧T10～L1，L3～L5，L7～S2背根节，保留L2和L5背极为备用背根。术后五天取手术侧和非手术侧T9～S3节段脊髓背角组织，分别匀浆、离心后，所得上清液即为提取液。提取液经SephadexG－200凝胶过滤层析后，获得三个主峰，分别收集手术侧和非手术侧三个峰值处的洗脱液，透析、冻干，用再改良的悬滴培养法进行生物活性检测。结果发现：手术侧背角提取液中第二峰值洗脱液促鸡胚背根节神经突起生长的作用明显张于非手术侧者（Pwto．01），在加入量为50ug／ml时，促神经突起生长作用最强。本研究结果为进一步分离纯化该神经营养活性物质奠定了基础。
In the present study, we found that the resection of the spinal cord plasticity has confirmed that the retained dorsal root (spare) fibers can be used to compensate the severed afferent fibers and to reconstruct their spinal cord (dorsal horn) Touch contact. Electrophoresis and in vitro culture methods also found that the spare dorsal root cats to the afferent side spinal cord dorsal horn tissue extract some of the changes in the content of the protein, and its promotion of neurite outgrowth role. Based on the above studies, we use gel filtration chromatography to separate the relevant proteins in the extract that promote neurite outgrowth. Thirty adult male domestic cats were used in the experiment. T10 ~ L1, L3 ~ L5 and L7 ~ S2 dorsal root segments were excised on one side, while the dorsal roots of L2 and L5 were reserved as spare dorsal roots. Five days after operation, the spinal dorsal horn tissues of T9-S3 segments of the operative and non-surgical sides were homogenized and centrifuged, respectively. The resulting supernatant was extracted. After the extract was separated by gel filtration chromatography on Sephadex G-200, three main peaks were obtained. The three peak elution fractions on the surgical side and the non-surgical side were collected, dialyzed and freeze-dried respectively. Activity test. The results showed that: the second peak elution of the surgical dorsal horn extract to promote chick embryo dorsal rhizome neurite outgrowth Zhang was significantly in the non-operation side (Pwto.01), at a dosage of 50ug / ml, the pro- The strongest growth effect. The results of this study laid the foundation for further separation and purification of the neurotrophic active substance.