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研究了金龟子绿僵茵(Metarhiziumanisopliae)在土壤中的发育过程、数量消长及控制桃小食心虫的潜力.结果表明,接种62~82d后所有处理的CFU均降低80%以上,121~234d后降低99%以上.在灭菌和不灭菌土壤中,分生孢子初始接种量的半衰期分别为28.5d和23.8d,干菌丝粉分别为26.9d和19.6d.在土壤接种后100d,将桃小食心虫冬茧接入土壤,冬茧被侵染产孢后补偿了带菌量的下降,278dCFU比不接冬茧土壤第271d的带菌量大1000倍.在土壤接种后,不同时间接入土壤的冬茧死亡率直到第131d还保持在90%以上,但到第237d降至9.3%.未用绿僵菌处理的土壤中接入的桃小食心虫冬茧无死亡发生.小区试验中,在15、22.5、30和37.5kg·hm(-2)分生抱孢制剂剂量下死亡率达97.0~100%,而蛀果率仅为2.7~5.0%.在陕北进行了大田试验面积达670hm2,剂量为22.5kg·hm(-2)分生孢子制剂.蛀果率降至2.4%,未处理果园则高达30%.
The development process, quantity and growth of Metarhiziumanisopliae in soil and its potential for controlling peach moth were studied. The results showed that the CFU of all treatments decreased by more than 80% after 62 ~ 82d and decreased by 99% after 121 ~ 234d. The half-lives of the initial inoculum inocula were 28.5d and 23.8d, respectively, in the sterile and non-sterile soil, and that of the dried mycelia were 26.9d and 19.6d respectively. 100 d after soil inoculation, the peach moth cocoon access to soil, winter cocoons were infected spores after inoculation to compensate for the decline in the amount of bacteria, 278dCFU than the winter cocoon soil No. 271d carrying capacity 1000 times larger. After inoculation of soil, the mortality of winter cocoon fed into soil at different times remained above 90% until 131 days, but decreased to 9.3% by 237th days. Pest insects access to the soil without Metarhizium anisopliae cocoon no death occurred. In the plot test, the mortality rate reached 97.0-100% at doses of 15, 22.5, 30 and 37.5 kg · hm -2 (-2), while the fruit-boring rate was only 2.7-5 .0%. Field experiments in northern Shaanxi field area of 670hm2, a dose of 22.5kg · hm (-2) conidia preparation. The fruit bore rate dropped to 2.4%, while the untreated orchard up to 30%.