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目的:研究复方藤梨根制剂(FFTLG)对人胃癌裸鼠腹腔移植瘤的抑制效应及其作用机制。方法:建立人胃癌(SGC-7901)裸鼠腹腔移植模型。24只裸鼠随机分为4组:FFTLG高、中、低剂量组和生理盐水对照组,分别观察荷瘤裸鼠腹腔移植瘤的生长情况,免疫组化检测肿瘤基质金属蛋白酶-9(MMP-9)的表达情况。结果:NS组、FFTLG低、中、高剂量组抑瘤率分别为0、7.8%、30.0%和33.3%;实验组与模型对照组相比具有统计学意义(P<0.05,P<0.01);腹腔瘤组织中MMP-9阳性表达细胞百分比分别为(56.13±23.26)%、(45.83±13.40)%、(30.67±11.50)%、(17.53±12.50)%。实验组与模型对照组相比具有统计学意义(P<0.05,P<0.01)。结论:FFTLG对裸鼠腹腔移植瘤生长具有抑制作用,且随着剂量的增加其作用增强,其作用机制可能与下调MMP-9的表达有关。
Objective: To study the inhibitory effect and its mechanism of Compound Tengligen (FFTLG) on human gastric cancer xenografts in nude mice. Methods: The model of human gastric cancer (SGC-7901) transplanted into abdominal cavity of nude mice was established. Twenty-four nude mice were randomly divided into 4 groups: high, medium and low dose FFTLG group and saline control group, respectively, to observe the growth of transplanted tumor in nude mice transplanted tumor, immunohistochemical detection of tumor matrix metalloproteinase-9 (MMP- 9) expression. Results: The tumor inhibition rates of NS, FFTLG low, medium and high dose groups were 0, 7.8%, 30.0% and 33.3%, respectively. There was statistical significance between the experimental group and the model control group (P <0.05, P <0.01) ; The percentage of MMP-9 positive cells in peritumor tissues was (56.13 ± 23.26)%, (45.83 ± 13.40)%, (30.67 ± 11.50)% and (17.53 ± 12.50)%, respectively. The experimental group compared with the model control group was statistically significant (P <0.05, P <0.01). CONCLUSION: FFTLG can inhibit the growth of transplanted abdominal tumor in nude mice, and its effect is enhanced with the increase of dose. The mechanism may be related to the down-regulation of MMP-9 expression.