论文部分内容阅读
目的研究纳米四氧化三铁(Fe3O4)染毒对小鼠肝、肾、脑组织损伤的病理表现。方法昆明小鼠40只随机分为生理盐水阴性对照组、纳米Fe3O4的高剂量组、中剂量组和低剂量组,染毒剂量分别为2、1和0.5 mg/kg,腹腔注射染毒,隔天1次,2周后取其肝、肾、脑组织,制作病理切片,观察其病理变化。结果对照组小鼠肝、肾、脑组织结构未见明显变化;各染毒组小鼠肝组织发生不同程度的肝细胞索排列紊乱,细胞边界不清,且高剂量组出现枯死细胞;各染毒组肾组织细胞胞浆不均匀,远曲小管管腔不规则,部分肾小球出现萎缩,高剂量组小鼠肾小管管腔狭窄,小管上皮细胞肿胀,管腔内有粉染絮状物;各染毒组脑组织未见异常。结论纳米Fe3O4染毒可致小鼠肝、肾组织损伤。
Objective To study the pathological manifestation of liver, kidney and brain injury induced by nano-Fe3O4 in mice. Methods Forty Kunming mice were randomly divided into saline control group, high-dose group of nano-Fe3O4, medium-dose group and low-dose group. The dose of the drug was 2, 1 and 0.5 mg / kg, Day 1, 2 weeks after the liver, kidney and brain tissue were taken and made pathological sections to observe the pathological changes. Results There was no significant change in the structure of liver, kidney and brain in the control group. The hepatic tissue of mice in each treatment group had disorder of hepatic cell arrangement, unclear cell boundary and dead cells in high dose group. Toxicity of the cytoplasm of renal tissue cells are uneven, irregular distal tubule irregularities, part of the glomerular atrophy, high-dose group of renal tubular stenosis, tubular epithelial cells swollen, lumens in the lumen with flocculent No abnormalities were found in the brain tissues of each exposure group. Conclusion Nano-Fe3O4 can cause liver and kidney damage in mice.