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在28℃、33℃两种温度下,用海豚链球菌人工感染尼罗罗非鱼后,测定尼罗罗非鱼血液生化指标。结果显示:两个温度水平病鱼的血清总蛋白(TP)随时间延长都呈现升高趋势;谷丙转氨酶(ALT)、谷草转氨酶(AST)活性随时间延长先升高后降低再升高,33℃实验组ALT、AST活性比28℃实验组达峰值时间短,并显著高于对照组(p<0.05);尿素、钾(K+)含量随时间延长逐渐升高后降低,钠(Na+)含量先升高后降低再升高,肌酐含量呈上升趋势;血清碱性磷酸酶(AKP)活性28℃实验组呈先升高后降低趋势,33℃实验组呈升高趋势;超氧化歧化酶(SOD)活性随时间延长先升高后降低再升高,33℃实验组各个时间点基本高于28℃实验组;球蛋白(GLO)随感染后时间延长呈现升高趋势,至120 h,显著高于对照组(p<0.05),但温度组间各时间点没有显著差异(p>0.05)。组织病理学观察发现,33℃实验组肝细胞、肾小管上皮细胞水肿变性;28℃实验组肝细胞出现嗜酸性小体;肾小管上皮细胞出现嗜酸性的红色小体,颗粒变性。表明感染海豚链球菌的尼罗罗非鱼肝脏、肾脏均有损伤,造成功能障碍,高温时损伤更严重。
The blood biochemical indexes of Nile tilapia (Nile tilapia) were determined by artificial infection of Nile tilapia at 28 ℃ and 33 ℃. The results showed that the serum total protein (TP) of diseased fish at both temperature levels showed an increasing trend over time; the activity of ALT and AST increased at first, then decreased, then increased, The activities of ALT and AST in the experimental group at 33 ℃ were shorter than those in the experimental group at 28 ℃ and significantly higher than those in the control group (p <0.05). The content of urea and potassium increased gradually and then decreased with time prolonging, The content of creatinine increased first and then decreased, and creatinine content increased. The serum AKP activity increased at first and then decreased at 28 ℃, and increased at 33 ℃ in experimental group. The activity of superoxide dismutase (SOD) activity increased first, then decreased, and then increased again. The 33 ℃ experimental group was higher than the 28 ℃ experimental group at each time point. The globulin (GLO) increased with the prolongation of infection time. After 120 h, (P <0.05), but there was no significant difference between temperature groups at each time point (p> 0.05). Histopathological observation showed that edema and degeneration of hepatocytes and tubular epithelial cells were observed in the experimental group at 33 ℃. Eosinophils were found in the hepatocytes of the experimental group at 28 ℃. Eosinophilic red bodies and granular degeneration appeared in the renal tubular epithelial cells. The results showed that the liver and kidney of Nile tilapia infected with S. iniae were damaged, causing dysfunction and more serious damage at high temperature.