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近年来对茶碱血浓度的测定方法研究颇多,国内多采用紫外分光光度法,亦有采用荧光分光光度法。紫外法虽然操作简便,设备要求低,但由于其专一性较差,红霉素、磺胺类、激素类药物均可干扰茶碱的测定;而且紫外法取血量较大,需从静脉采血,难以为病人所接受,因而临床应用常常受到限制。本文介绍一种快速、灵敏、准确的高效液相色谱法,本法操作简便,专一性好,哮喘病人常合用的10几种药物均无干扰。由手指采血0.1ml即可,病人容易接受,具有较好的临床实用性。
In recent years, the determination of theophylline blood concentration of a lot of research methods, the domestic use of UV spectrophotometry, but also the use of fluorescence spectrophotometry. Although the UV method is easy to operate and has low equipment requirements, due to its poor specificity, erythromycin, sulfonamides and hormone drugs can interfere with the theophylline determination. Moreover, the UV method requires a large amount of blood to be taken from venous blood , Difficult for the patient to accept, so clinical applications are often limited. This article describes a fast, sensitive, accurate high performance liquid chromatography, the method is simple, good specificity, asthma patients commonly used 10 kinds of drugs are not interfering. By the finger blood 0.1ml, the patient is easy to accept, with good clinical practicability.