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目的：介绍一种同时评估耳蜗传出神经和毛细胞的简便的组织化学技术。方法：首先应用脱氢酶染色选择性标记毛细胞，再用乙酰胆碱酯酶染色标记传出神经纤维。双重染色的耳蜗铺片样品在光学显微镜下沿着耳蜗基底膜的全长分别对毛细胞和穿越Ｃｏｒｔｉ隧道的传出神经纤维计数；根据卡铂耳中毒灰鼠的毛细胞及其传出神经纤维损伤的百分比制备耳蜗图。结果：耳蜗分析图充分显示：当绝大多数内毛细胞坏死以及部分外毛细胞坏死时，穿越隧道的传出神经纤维数的减少接近于外毛细胞损失的百分比；但当大量内毛细胞坏死而外毛细胞正常时，穿越隧道的传出神经纤维亦保持正常。结论：卡铂损害耳蜗传出神经系统可能是由于外毛细胞受损所致。
Objective: To introduce a simple histochemical technique for simultaneous assessment of cochlear efferent nerve and hair cells. Methods: The hair cells were selectively labeled with dehydrogenase staining, and then the nerve fibers were labeled with acetylcholinesterase staining. Double-stained cochlear implants samples were counted under light microscopy along the entire length of the cochlear basement membrane on hair cells and the outgoing nerve fibers that traverse the Corti tunnel. Based on the hair cells of the squirrel and its afferent nerve fibers Cochlear implants were prepared as a percentage of lesions. Results: The cochlear analysis showed that when most of the inner hair cells were necrotic and part of the outer hair cells were necrotic, the decrease of the number of exiting nerve fibers crossing the tunnel was close to the percentage of the loss of outer hair cells; however, when a large number of inner hair cells were necrotic While the normal outer hair cells, the outgoing nerve fibers through the tunnel also remained normal. CONCLUSION: Carboplatin damage to the cochlear nervous system may be due to impaired outer hair cells.