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本文比较研究了新鲜和冷冻保存的骨髓(BM)标本的长期培养(HLTBMCs),测定了活体及尸体BM短期和长期培养时的生长情况。骨髓来自正常供髓者和脑疾病死亡的尸体,采到的骨髓混悬于含50%胎牛血清,40%1640介质液和10%DMSO的液体中,在液氮蒸气中过夜,然后移在液氮中保存。用37℃水浴融化后的BM与含有0.067mg/ml牛胰腺I和5mM的MgSO_4的胎牛血清孵育10分钟,1640洗涤后用台盼蓝计算细胞排染率,同时做细胞短期和长期培养,与未经冷冻的BM比较CFU-GM生长及非贴壁细胞不同类型数目的变化。结果未经冷冻的尸体BM短期培养后CFU-GM低于活体BM。可能是由于患者死亡前皮质类固醇的注入使成熟粒细胞增加而稀释了
In this study, long-term culture (HLTBMCs) of freshly and cryopreserved bone marrow (BM) specimens were compared to determine the short and long term growth of live and dead BM. Bone marrow was from a normal corpse and died from brain disease. The harvested bone marrow was suspended in a liquid containing 50% fetal bovine serum, 40% 1640 medium, and 10% DMSO overnight in liquid nitrogen vapor before moving Preserved in liquid nitrogen. After thawing in a 37 ° C water bath, BMs were incubated with fetal bovine serum containing 0.067 mg / ml bovine pancreas I and 5 mM MgSO 4 for 10 minutes. After 1640 washing, cell staining rates were calculated by trypan blue, short-term and long- CFU-GM growth and number of different types of non-adherent cells were compared to non-frozen BM. Results CFU-GM was lower than BM after short-term culture of non-frozen corpses. Possibly due to an increase in mature granulocytes with dilution of pre-corticosteroid infusion