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目的：比较伯氏疟原虫氯喹抗性（ＲＣ）株和敏感（Ｎ）株对宿主肝脏细胞色素Ｐ４５０ⅡＢ１基因ｍＲＮＡ转录影响的差异。方法：以小鼠ＣＹＰ４５０ⅡＢ１基因的ｃＤＮＡ（５５２ｂｐ）为探针，分别与感染伯氏疟原虫ＲＣ株和Ｎ株的小鼠肝脏总ＲＮＡ进行Ｎｏｒｔｈｅｒｎｂｌｏｔ杂交。结果：感染伯氏疟原虫Ｎ株小鼠肝ＣＹＰ４５０ⅡＢ１基因的ｍＲＮＡ明显低于感染ＲＣ株鼠及正常对照鼠，而感染ＲＣ株鼠与正常对照鼠之间该基因的转录无明显差异。结论：伯氏疟原虫ＲＣ株和Ｎ株感染对宿主肝脏ＣＹＰ４５０ⅡＢ１基因ｍＲＮＡ转录的影响有显著差异，为氯喹抗药性疟原虫可加速宿主氯喹代谢的抗药性假说提供了分子生物学依据
OBJECTIVE: To compare the effects of chloroquine resistant (RC) and sensitive (N) strains of Plasmodium berghei on mRNA transcription of cytochrome P450 Ⅱ B1 in liver. Methods: The cDNA of mouse CYP450ⅡB1 gene (552bp) was used as probe to Northern blotting with the total RNA of liver of mice infected with Plasmodium berghei RC strain and strain N respectively. Results: The mRNA of liver CYP450ⅡB1 in mice infected with Plasmodium berghei was significantly lower than that in RC strains and normal control mice. There was no significant difference in the transcription of this gene between infected RC strains and normal control mice. Conclusion: The effects of Plasmodium berberine RC strain and strain N on the transcription of CYP450ⅡB1 mRNA in host liver were significantly different, which provided a molecular biological basis for the resistance hypothesis that chloroquine resistant Plasmodium could accelerate the host chloroquine metabolism