论文部分内容阅读
Objectives To determine whether morphine induced a preconditioning effect in endothelial cells. Methods Human umbilical vein endothelial cells (HUVEC) were subjected to oxygen-glucose deprivation (OGD) to simulate ischemia in vitro. Cell viability was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. Results OGD time-dependently decreased the HUVEC viability. Morphine pretreatment applied 5 min or 24 hr before a 20-hr OGD dose-dependently attenuated the OGD-reduced cell viability. The EC50 for this effect was 1.84 and 0.26 μM, respectively, for morphine applied 5 min or 24 hr before the OGD. Conclusion morphine induces ischemic tolerance in endothelial cells.
Objectives To determine whether morphine induced a preconditioning effect in endothelial cells. Methods Human umbilical vein endothelial cells (HUVEC) were subjected to oxygen-glucose deprivation (OGD) to simulate ischemia in vitro. Cell viability was evaluated by the 3- (4, 5 -Dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide assay. OGD time-dependently decreased the HUVEC viability. Morphine pretreatment applied 5 min or 24 hr before a 20-hr OGD dose-dependently attenuated the OGD-reduced cell viability . The EC50 for this effect was 1.84 and 0.26 μM, respectively, for morphine applied 5 min or 24 hr before the OGD. Conclusion Morphine induces ischemic tolerance in endothelial cells.