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肺表面活性蛋白A(SP-A)是一种肺特有的磷脂相关性糖蛋白.因为目前认为只有肺腺癌产生SP-A,本文就胸水SP-A水平诊断肺腺癌的特异性和实用性进行了研究.材料与方法:恶性肿瘤94例和肺结核7例.恶性肿瘤包括肺腺癌67例,肺鳞癌9例,小细胞癌8例,不同原发部位的腺癌10例(胃4例,结肠2例,乳腺2例,胰腺、卵巢各1例).采集胸水标本离心后取上清液以ELISA法检测S-PA,并进行蛋白印迹分析.结果:肺腺癌、鳞癌、小细胞肺癌、其他部位腺癌和肺结核的胸水平均SP-A含量分别为2421.4±822.6ng/ml,181.1±35.6ng/ml,276.6±55.0ng/ml,150.3±24.3ng/ml和126.4±22.8ng/ml.胸水SP-A水平超过
Pulmonary surfactant protein A (SP-A) is a lung-specific phospholipid-related glycoprotein. Because it is currently considered that lung adenocarcinoma produces SP-A, the specificity and utility of pleural effusion SP-A in the diagnosis of lung adenocarcinoma Sex was studied. Materials and methods: 94 cases of malignant tumors and 7 cases of pulmonary tuberculosis. Malignant tumors included 67 cases of lung adenocarcinoma, 9 cases of squamous cell carcinoma of the lung, 8 cases of small cell carcinoma, and 10 cases of adenocarcinoma of different primary sites (stomach). 4 cases, 2 cases of colon, 2 cases of mammary gland, 1 case of pancreas and 1 case of ovary). The pleural effusion specimens were collected and centrifuged to take supernatants. S-PA was detected by ELISA and Western blot analysis was performed. Results: Lung adenocarcinoma, squamous cell carcinoma The mean SP-A levels of pleural fluid in squamous cell lung cancer, adenocarcinoma of other sites, and tuberculosis were 2421.4±822.6 ng/ml, 181.1±35.6 ng/ml, 276.6±55.0 ng/ml, 150.3±24.3 ng/ml, and 126.4±, respectively. 22.8 ng/ml. SP-A level in pleural effusion exceeded