Objective:To evaluate the rate and types of human papillomavirus(HPV)infection in cervical adenocarcinoma.Methods:We detected and identified HPV in 67 lesions using PCR based reverse line blot hybridization and DNA sequencing.Among the 67 patients,53 were diagnosed as cervical adenocarcinoma and 14 as cervical adenosquamous carcinoma.First a fragment of 150 bp was amplified from the L1 region of HPV with GP5/GP6 primers.If the result was negative,a short fragment of 65 bp was amplified from the L1 region with SPFI/SPF2 primers.Results:6 cases were eliminated from the study because of unsatisfied DNA extraction.The total positive rate of HPV DNA detected by PCR in cervical adenocarcinoma and adenosquamous carcinoma was 91.8%(56/61).Using general primer GP5/GP6,the positive rate was 50.8%(31/61).Using SPF primers for the 30 negative cases,25 additional HPV positive cases were founded.All the positive samples had at least one of the high risk types.HPV16 was the most preferential type followed by HPV18,31,39 and 45.The infection of HPV 16 and 18 accounted for about half of HPV-positive adenocarcinoma.Multiple HPV infections were found in 21.4%(12/56)of the cases.Conclusion:The high risk type of HPV is associated with cervical adenocarcinoma.Single infection is more frequently presented than multiple infections,no single type of HPV plays a predominated role even HPV16 and 18 are the major types.