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目的多发性骨髓瘤(MM)是来源于B淋巴细胞的恶性肿瘤,以终末分化的浆细胞的克隆性增生为显著特征。骨质破坏是其突出的临床特点之一,与疾病的预后直接相关。多种细胞因子参与了骨质破坏的过程。加强对MM骨病机制的研究将为临床靶向治疗和改善患者生存质量提供更好的依据。本研究旨在探讨神经元特异性烯醇化酶(NSE)在骨髓瘤骨病中的作用。方法采用ELISA和实时荧光定量RT-PCR方法,对MM患者血浆和骨髓中以及骨髓瘤细胞株U266中NSE水平进行定量分析,初步分析其在骨髓微环境中的表达情况,了解其与MM骨病的关系。结果U266和67%的患者中NSE水平明显高于对照组(P<0.01),且随着临床分期和骨质破坏程度的增加,NSE表达总体呈上升趋势。结论 U266和大部分骨髓瘤骨病患者中NSE表达水平增高,且与MM骨病程度和疾病分期相关,提示NSE可能在骨髓瘤骨病中起重要作用。
Purpose Multiple myeloma (MM) is a malignant tumor derived from B lymphocytes characterized by clonal hyperplasia of terminally differentiated plasma cells. Bone destruction is one of the prominent clinical features, directly related to the prognosis of the disease. A variety of cytokines involved in the process of bone destruction. To strengthen the study of the mechanism of MM bone disease will provide a better basis for clinical targeted therapy and improve the quality of life of patients. This study aimed to investigate the role of neuron-specific enolase (NSE) in myeloma bone disease. Methods The levels of NSE in plasma and bone marrow of MM patients and myeloma cell line U266 were quantitatively analyzed by ELISA and real-time fluorescence quantitative RT-PCR. The expression of NSE in bone marrow micro-environment was analyzed preliminarily to investigate the relationship between NSE level and MM bone disease Relationship. Results NSE levels in U266 and 67% of patients were significantly higher than those in control group (P <0.01). With the increase of clinical stage and the degree of bone destruction, NSE expression showed an overall upward trend. Conclusion The expression of NSE in U266 and most myeloma patients is higher than that in patients with MM and is related to the degree and stage of MM. It suggests that NSE may play an important role in myeloma.