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利用 16对水稻功能基因的SSR引物研究了 2 3份世界 5个国家不同来源的水稻种质资源的遗传多样性 ,共检测出 78个等位基因变异 ,每对引物可检测 2～ 10个等位基因变异 ,平均为 5 .2个 ,品种间遗传相似系数在0 .13～ 0 .6 4之间。UPGMA聚类分析表明 ,在相似系数 0 .13处可以将材料分为 2大类 ,来自巴西、日本和中国的粳稻全部聚为第Ⅰ类 ,巴西陆稻和原产科特迪瓦的陆稻聚在第Ⅰ类的第三小群 ;第Ⅱ类全为籼稻 ,来源于巴基斯坦的籼稻和 1个来源于韩国的籼稻分布在第Ⅱ类 ,说明水稻功能基因在不同亚种、不同产地来源和不同生态类型的水稻之间存在差异 ,也进一步证实水稻功能基因的SSR标记是研究水稻种质资源分类、地理分布、生态类型和系谱分析的有效工具。
Genetic diversity of 23 rice germplasm resources from 5 different countries in the world was studied using 16 pairs of SSR primers of rice functional genes. A total of 78 alleles were detected, of which 2 to 10 The average number of alleles was 5.2, and the genetic similarity coefficient was 0.13 ~ 0.64. UPGMA cluster analysis showed that the materials could be divided into two groups at the similarity coefficient of 0.13. All of the japonica rice from Brazil, Japan and China were clustered into the first group, while the Upland rice from Brazil and the indigenous Ivorian rice were clustered in the The third subgroup of Class I; the second group is indica rice, the indica rice from Pakistan and one indica rice from South Korea are distributed in Class II, indicating that the functional genes of rice in different subspecies, different origin and different ecological types Of rice, and further confirmed that rice SSR markers of functional genes is an effective tool to study rice germplasm classification, geographical distribution, ecological type and pedigree analysis.