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目的:建立正畸牙根吸收动物模型,研究多西环素(DOXY)对根吸收组织的影响。方法:选用SD大鼠36只建立正畸牙移动根吸收动物模型,随机分为空白对照组(A组)、注射DOXY对照组(B组)、正常牙加力组(C组)、注射DOXY加力组(D组)。在加力的0、7、10、14 d分别处死各组大鼠。并取相应时段的标本制作组织切片,进行HE染色和TRAP染色,检测多西环素对根吸收组织的影响。用计算机图像分析技术定量比较上述实验结果。结果:1在所有实验周期内(除第14 d),D组根吸收明显少于C组(P<0.05)。D组根吸收的平均值缓慢增加,而C组14 d时显示减少。2C组、D组牙根面的TRAP阳性细胞数均在第10 d到达峰值,第14 d开始下降。然而D组在受力10 d后根面的TRAP阳性细胞数明显少于C组(P<0.05)。结论:多西环素可能对正畸牙根吸收及破骨细胞的募集有抑制作用。
OBJECTIVE: To establish an animal model of root resorption by orthodontics to study the effect of doxycycline (DOXY) on root resorption. Methods: Thirty-six SD rats were used to establish the animal model of root resorption by orthodontic tooth and randomly divided into blank control group (A group), DOXY injection group (B group), normal dental compression group (C group) and DOXY Afterburner group (group D). Rats were sacrificed at 0, 7, 10, 14 d after energized. Tissue sections of the specimens were made at the corresponding time points, HE staining and TRAP staining were performed to examine the effect of doxycycline on root tissue uptake. Computer image analysis techniques to quantitatively compare the above experimental results. Results: 1 In all experimental periods (except the 14th day), the root resorption of group D was obviously less than that of group C (P <0.05). The average value of root resorption in group D increased slowly, but decreased in group C on the 14th day. The numbers of TRAP positive cells in the root surface of group 2C and group D both reached the peak on the 10th day and decreased on the 14th day. However, the number of TRAP-positive cells in root surface of group D was significantly less than that of group C after 10 days stress (P <0.05). Conclusion: Doxycycline may inhibit the orthodontic root resorption and the recruitment of osteoclasts.