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目的探讨三叶因子3(Trefoil Factor 3,TFF3)、细胞外调节蛋白激酶2(Extracellular Regulated Protein Kinases 2,ERK2)及其磷酸化状态(p-ERK1/2)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中的表达水平和临床意义。方法采用Western Blot和免疫组织化学法检测60例甲状腺乳头状癌组织,30例甲状腺腺瘤组织(adenoma,A),30例瘤旁正常甲状腺组织(normal group,N)TFF3、ERK2、p-ERK1/2蛋白的表达情况,分析三种指标在甲状腺乳头癌中的表达阳性率与其临床病理特征的关系。结果 Western Blot结果显示TFF3、ERK2、p-ERK1/2蛋白在甲状腺乳头状癌组织表达水平均高于正常组和腺瘤组(P<0.05)。免疫组化结果显示TFF3、ERK2、p-ERK1/2阳性蛋白呈棕黄色颗粒,TFF3主要表达于胞浆,ERK2为胞浆(膜)阳性,p-ERK1/2以胞核阳性为主,部分细胞胞浆阳性。TFF3、ERK2、pERK1/2在甲状腺乳头状癌中阳性表达率分别为85.0%、81.7%、78.3%,明显高于腺瘤组和正常组(p<0.05),并与病理分期和淋巴结转移密切相关(P<0.05),TFF3与ERK2、p-ERK1/2的表达呈正相关(r=0.415,r=0.467,P<0.01)。结论 TFF3可能通过ERK通路促进了甲状腺乳头状癌的发展及转移。
Objective To investigate the expression of Trefoil factor 3 (TFF3), extracellular regulated protein kinase 2 (ERK2) and its phosphorylation status (p-ERK1 / 2) in papillary thyroid carcinoma , PTC) in the expression level and clinical significance. Methods Thirty cases of thyroid papillary carcinoma, 30 cases of adenoma (A), 30 cases of normal group (N) TFF3, ERK2 and p-ERK1 were detected by Western Blot and immunohistochemistry. / 2 protein expression, analysis of the three indicators in papillary thyroid carcinoma in the positive rate and its clinicopathological features. Results The results of Western Blot showed that the expression of TFF3, ERK2 and p-ERK1 / 2 in thyroid papillary carcinoma tissues were significantly higher than those in normal and adenoma groups (P <0.05). Immunohistochemistry results showed that TFF3, ERK2, p-ERK1 / 2 positive protein was brown granules, TFF3 mainly expressed in the cytoplasm, ERK2 cytoplasm (membrane) positive p-ERK1 / 2 nucleus positive, part Cytoplasm positive. The positive rates of TFF3, ERK2 and pERK1 / 2 in papillary thyroid carcinoma were 85.0%, 81.7% and 78.3%, respectively, which were significantly higher than those in adenoma group and normal group (p <0.05), and were closely correlated with pathological stage and lymph node metastasis (P <0.05). There was a positive correlation between TFF3 and ERK2 and p-ERK1 / 2 (r = 0.415, r = 0.467, P <0.01). Conclusion TFF3 may promote the development and metastasis of thyroid papillary carcinoma through ERK pathway.