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目的:乳腺癌是女性最常见的恶性肿瘤之一,并且有发病年龄提早的趋向,因此研究促进乳腺癌发生的因素显得尤为重要。HOXB7是同源异型盒基因家族中新近发现的一种转录因子,它在生理状态下的表达对维持正常细胞的生长和分化有非常重要的作用,然而其异常表达时可能促进肿瘤形成。本实验探讨HOXB7在乳腺癌组织中的表达及其临床意义。方法::收集正常乳腺组织20例、乳腺纤维瘤组织20例、癌组织60例,应用实时荧光定量逆转录聚合酶链反应(Real-time RT-PCR)检测三种乳腺组织中HOXB7 mR-NA的表达;应用Western Blotting法检测三种乳腺组织中HOXB7蛋白的表达水平
OBJECTIVE: Breast cancer is one of the most common malignant tumors in women and has an earlier age of onset. Therefore, it is particularly important to study the factors that promote the occurrence of breast cancer. HOXB7 is a newly discovered transcription factor in the homeobox gene family. Its expression in physiological state plays a very important role in maintaining the growth and differentiation of normal cells. However, it may promote tumor formation when abnormally expressed. This study explored the expression of HOXB7 in breast cancer and its clinical significance. Methods: Twenty normal breast tissues, 20 breast cancer tissues and 60 cancer tissues were collected. Real-time RT-PCR was used to detect HOXB7 mR-NA The expression of HOXB7 protein in three breast tissues was detected by Western Blotting