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本文建立了用毛细管电泳测定中草药芦根和蒲黄多糖中的单糖组分的方法。提取的单糖经α-萘胺衍生,用二极管阵列检测器在214nm处检测。五种单糖鼠李糖(Rha),木糖(Xyl),半乳糖(Gal),葡萄糖(Glu),阿拉伯糖(Ara)能够同时测定,分离良好。毛细管电泳的测定条件如下,50 mM硼酸钠缓冲液;pH10.5;工作电压为25kV;温度为25℃。标准曲线的线性范围为5 mg/L-200 mg/L,每种单糖的最低检测线均为2.5 mg/L。迁移时间和峰面积的相对标准偏差分别小于1.46%,6.21%。实验结果显示,芦根多糖重各单糖组分的摩尔比如下:木糖∶葡萄糖∶阿拉伯糖∶半乳糖为1.00∶59.03∶2.09∶1.96;蒲黄多糖中各单组分的摩尔比如下:鼠李糖∶木糖∶葡萄糖∶阿拉伯糖∶半乳糖为1.71∶1.00∶9.30∶6.88∶1.24。
In this paper, a method for the determination of monosaccharide components in Chinese reed rhizome and puffer polysaccharides by capillary electrophoresis was established. The extracted monosaccharides were derivatized with α-naphthylamine and detected with a diode array detector at 214 nm. The five single sugars Rha, Xyl, Gal, Glu and Ara could be determined simultaneously and separated well. The conditions for the capillary electrophoresis were as follows: 50 mM sodium borate buffer; pH 10.5; operating voltage 25 kV; temperature 25 ° C. The calibration curve has a linear range of 5 mg / L to 200 mg / L and the lowest detection limit for each monosaccharide is 2.5 mg / L. The relative standard deviations of migration time and peak area were less than 1.46% and 6.21% respectively. Experimental results show that the reed rhizome polysaccharide heavy monosaccharide components molar ratio as follows: xylose: glucose: arabinose: galactose 1.00: 59.03: 2.09: 1.96; Puhuang polysaccharide in the molar ratio of the individual components are as follows: Rhamnose: xylose: glucose: arabinose: galactose 1.71: 1.00: 9.30: 6.88: 1.24.