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通过流式细胞仪的方法，报道了凝集素美洲商陆（ＰＷＭ）对人单核细胞和白血病细胞表面的ＨＬＡ－ＤＲ分子表达的影响。实验结果显示：ＰＷＭ可以直接与单核细胞和白血病Ｕ９３７细胞及Ｒａｊｉ细胞相结合，而且这３种细胞均表达ＨＬＡ－ＤＲ分子。经ＰＷＭ处理２４ｈ后，正常人单核细胞ＨＬＡ－ＤＲ分子的表达不受影响，而Ｕ９３７细胞和Ｒａｊｉ细胞上的ＨＬＡ－ＤＲ分子均被下调，而且这种下调作用与ＰＷＭ的剂量有关。其他凝集素，如ＰＨＡ、ＷＧＡ和内毒素ＬＰＳ对白血病细胞ＨＬＡ－ＤＲ分子的表达无影响。结果表明，通过凝集素ＰＷＭ的处理可以改变肿瘤细胞表面ＭＨＣⅡ类分子的表达，从而为肿瘤免疫治疗提供新的途径。
The effect of lectin, pokeweed, on the expression of HLA-DR molecules on the surface of human monocytes and leukemia cells was reported by flow cytometry. Experimental results show that: PWM can be directly associated with monocytic and leukemic U937 cells and Raji cells, and all three cells express HLA-DR molecules. After treated with PWM for 24 h, the expression of HLA-DR in normal human monocytes was not affected, while the HLA-DR molecules in U937 cells and Raji cells were downregulated, and this down-regulation was related to the dose of PWM. Other lectins, such as PHA, WGA and LPS, had no effect on HLA-DR expression in leukemia cells. The results showed that the treatment of lectin PWM can change the expression of MHC class II molecules on the surface of tumor cells, thus providing a new way for tumor immunotherapy.